CLK2 blocking peptide

CLK2 Antibody (N-term) Blocking Peptide

• Synonyms: CLK2; CLK2 Antibody (N-term) Blocking Peptide; Dual specificity protein kinase CLK2; CDC-like kinase 2; CLK2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human CLK2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 499

• Cellular Location: Isoform 1: Nucleus. Nucleus speckle. Note: Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.
• Tissue Location: Endothelial cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CLK2 blocking peptide

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 1705919
• NCBI GeneID: 1196
• UniProt Accession #: P49760; Q96CQ0; B1AVS9; B5MBX6
• Molecular Weight: 59,962 Da
• NCBI Official Full Name: Dual specificity protein kinase CLK2
• NCBI Official Synonym Full Names: CDC like kinase 2
• NCBI Official Symbol: CLK2
• NCBI Protein Information: dual specificity protein kinase CLK2
• UniProt Protein Name: Dual specificity protein kinase CLK2
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK2
• UniProt Entry Name: CLK2_HUMAN

NCBI Description

This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

CLK2: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, dual-specificity (non-receptor); Protein kinase, CMGC; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; protein serine/threonine kinase activity

Biological Process: negative regulation of gluconeogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of RNA splicing; response to ionizing radiation