Merlin Blocking Peptide | NF2 blocking peptide

Merlin Antibody (N-term) Blocking Peptide

• Gene Names: NF2; ACN; SCH; BANF
• Synonyms: Merlin; Merlin Antibody (N-term) Blocking Peptide; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin; NF2; SCH; NF2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human Merlin. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 595

• Cellular Location: Isoform 1: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note: In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions Isoform 9: Cytoplasm, perinuclear region. Cytoplasmic granule. Note: Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia
• Tissue Location: Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NF2 blocking peptide

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

NCBI and Uniprot Product Information

• NCBI GI #: 462594
• NCBI GeneID: 4771
• UniProt Accession #: P35240; O95683; Q8WUJ2; Q969N0; Q969Q3; Q96T30; Q96T31; Q96T32; Q96T33; Q9BTW3; Q9UNG9; Q9UNH3
• Molecular Weight: 24,515 Da
• NCBI Official Full Name: Merlin
• NCBI Official Synonym Full Names: neurofibromin 2
• NCBI Official Symbol: NF2
• NCBI Official Synonym Symbols: ACN; SCH; BANF
• NCBI Protein Information: merlin
• UniProt Protein Name: Merlin
• Protein Family: Merlin
• UniProt Gene Name: NF2
• UniProt Synonym Gene Names: SCH
• UniProt Entry Name: MERL_HUMAN

NCBI Description

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Uniprot Description

Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Tumor suppressor

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cytoplasm; cytoskeleton; early endosome; membrane; nucleolus; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: protein binding

Biological Process: actin cytoskeleton organization and biogenesis; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of cell-matrix adhesion; negative regulation of DNA replication; negative regulation of JAK-STAT cascade; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of stress fiber formation; Schwann cell proliferation

Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1