PSMD11 blocking peptide

PSMD11 Antibody (C-term) Blocking Peptide

• Gene Names: PSMD11; S9; Rpn6; p44.5
• Synonyms: PSMD11; PSMD11 Antibody (C-term) Blocking Peptide; 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit p445; PSMD11 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human PSMD11. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 422

• Cellular Location: Nucleus. Cytoplasm, cytosol
• Tissue Location: Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PSMD11 blocking peptide

Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

NCBI and Uniprot Product Information

• NCBI GI #: 20978543
• NCBI GeneID: 5717
• UniProt Accession #: O00231; O00495; Q53FT5; A8K3I7; E1P663
• Molecular Weight: 47,535 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 11
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 11
• NCBI Official Symbol: PSMD11
• NCBI Official Synonym Symbols: S9; Rpn6; p44.5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 11
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 11
• UniProt Gene Name: PSMD11
• UniProt Entry Name: PSD11_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMD11: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Belongs to the proteasome subunit S9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytosol; membrane; nucleoplasm; nucleus; proteasome complex

Molecular Function: protein binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; proteasome assembly; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stem cell differentiation; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway