USP4 blocking peptide

USP4 Antibody (C-term) Blocking Peptide

• Gene Names: USP4; UNP; Unph
• Synonyms: USP4; USP4 Antibody (C-term) Blocking Peptide; Ubiquitin carboxyl-terminal hydrolase 4; Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog; UNP; UNPH; USP4 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human USP4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 963

• Cellular Location: Cytoplasm. Nucleus Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
• Tissue Location: Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for USP4 blocking peptide

Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.

NCBI and Uniprot Product Information

• NCBI GI #: 116242839
• NCBI GeneID: 7375
• UniProt Accession #: Q13107; O43452; O43453; Q08AK8; A8K6Y0; C9IY91
• Molecular Weight: 35,661 Da
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase 4
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 4
• NCBI Official Symbol: USP4
• NCBI Official Synonym Symbols: UNP; Unph
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 4
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 4
• UniProt Gene Name: USP4
• UniProt Synonym Gene Names: UNP; UNPH
• UniProt Entry Name: UBP4_HUMAN

NCBI Description

The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin-specific protease; Oncoprotein; EC 3.4.19.12; Protease

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasm; lysosome; nucleus; plasma membrane

Molecular Function: adenosine receptor binding; identical protein binding; protein binding; ubiquitin-specific protease activity

Biological Process: assembly of spliceosomal tri-snRNP; negative regulation of protein ubiquitination; protein deubiquitination; regulation of protein stability