APG4D (S341) Blocking Peptide | ATG4D blocking peptide

APG4D Antibody (S341) Blocking Peptide

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Synonyms: APG4D (S341); APG4D Antibody (S341) Blocking Peptide; Cysteine protease ATG4D; 3422-; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; mitochondrial; ATG4D; APG4D; AUTL4; ATG4D blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the S341 region of human APG4D. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 474

• Cellular Location: Cysteine protease ATG4D: Cytoplasm.
• Tissue Location: Mainly expressed in skeletal muscle and, to a lower extent, in testis.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATG4D blocking peptide

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

NCBI and Uniprot Product Information

• NCBI GI #: 61211809
• NCBI GeneID: 84971
• UniProt Accession #: Q86TL0; Q969K0
• Molecular Weight: 16,008 Da
• NCBI Official Full Name: Cysteine protease ATG4D
• NCBI Official Synonym Full Names: autophagy related 4D cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D
• UniProt Protein Name: Cysteine protease ATG4D
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4
• UniProt Entry Name: ATG4D_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.

Protein type: Protease; Autophagy; EC 3.4.22.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane