MCM7 blocking peptide

MCM7 Antibody (N-term) Blocking Peptide

• Gene Names: MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• Synonyms: MCM7; MCM7 Antibody (N-term) Blocking Peptide; DNA replication licensing factor MCM7; CDC47 homolog; P11-MCM3; CDC47; MCM2; MCM7 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 719

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MCM7 blocking peptide

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

NCBI and Uniprot Product Information

• NCBI GI #: 20981696
• NCBI GeneID: 4176
• UniProt Accession #: P33993; Q15076; Q96D34; Q96GL1; A4D2A1; A4D2A2; E9PGN9
• Molecular Weight: 60,643 Da
• NCBI Official Full Name: DNA replication licensing factor MCM7
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 7
• NCBI Official Symbol: MCM7
• NCBI Official Synonym Symbols: MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• NCBI Protein Information: DNA replication licensing factor MCM7
• UniProt Protein Name: DNA replication licensing factor MCM7
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM7
• UniProt Synonym Gene Names: CDC47; MCM2
• UniProt Entry Name: MCM7_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR- ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Belongs to the MCM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Motility/polarity/chemotaxis; DNA replication

Chromosomal Location of Human Ortholog: 7q21.3-q22.1

Cellular Component: chromatin; cytoplasm; MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm

Molecular Function: ATP-dependent DNA helicase activity; DNA binding; protein binding

Biological Process: DNA replication; G1/S transition of mitotic cell cycle; regulation of phosphorylation; response to DNA damage stimulus