Cdk2 blocking peptide

Mouse Cdk2 Antibody (C-term) Blocking Peptide

• Gene Names: Cdk2; A630093N05Rik
• Synonyms: Cdk2; Mouse Cdk2 Antibody (C-term) Blocking Peptide; Cyclin-dependent kinase 2; Cell division protein kinase 2; Cdkn2; Cdk2 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 346

• Cellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome Note: Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Cdk2 blocking peptide

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT- mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization.

NCBI and Uniprot Product Information

• NCBI GI #: 8039782
• NCBI GeneID: 12566
• UniProt Accession #: P97377; O55105
• Molecular Weight: 33,887 Da
• NCBI Official Full Name: Cyclin-dependent kinase 2
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2
• NCBI Official Symbol: Cdk2
• NCBI Official Synonym Symbols: A630093N05Rik
• NCBI Protein Information: cyclin-dependent kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: Cdk2
• UniProt Synonym Gene Names: Cdkn2
• UniProt Entry Name: CDK2_MOUSE

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Cell cycle regulation; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Cellular Component: Cajal body; chromosome, telomeric region; condensed chromosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; endosome; intracellular membrane-bound organelle; nucleus; transcription factor complex; X chromosome; Y chromosome

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; kinase activity; protein binding; protein complex binding; protein kinase activity

Biological Process: cellular response to insulin stimulus; centriole replication; G1/S transition of mitotic cell cycle; peptidyl-serine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA replication initiation; positive regulation of transcription, DNA-dependent; potassium ion transport; protein amino acid phosphorylation; response to cAMP; response to electrical stimulus