BANF1 blocking peptide

BANF1 Antibody (Center) Blocking peptide

• Gene Names: BANF1; BAF; NGPS; BCRP1; D14S1460
• Synonyms: BANF1; BANF1 Antibody (Center) Blocking peptide; Barrier-to-autointegration factor; Breakpoint cluster region protein 1; N-terminally processed; BAF; BCRG1; BANF1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of BANF1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 89

• Cellular Location: Nucleus. Cytoplasm. Chromosome. Note: Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization
• Tissue Location: Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for BANF1 blocking peptide

Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.

NCBI and Uniprot Product Information

• NCBI GI #: 5921173
• NCBI GeneID: 8815
• UniProt Accession #: O75531; O60558; Q6FGG7
• Molecular Weight: 10,059 Da
• NCBI Official Full Name: Barrier-to-autointegration factor
• NCBI Official Synonym Full Names: barrier to autointegration factor 1
• NCBI Official Symbol: BANF1
• NCBI Official Synonym Symbols: BAF; NGPS; BCRP1; D14S1460
• NCBI Protein Information: barrier-to-autointegration factor
• UniProt Protein Name: Barrier-to-autointegration factor
• Protein Family: Barrier-to-autointegration factor
• UniProt Gene Name: BANF1
• UniProt Synonym Gene Names: BAF; BCRG1
• UniProt Entry Name: BAF_HUMAN

NCBI Description

The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009]

Uniprot Description

BAF: Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Defects in BANF1 are the cause of Nestor-Guillermo progeria syndrome (NGPS). NGPS is an atypical progeroid syndrome characterized by normal development in the first years of life, later followed by the emergence of generalized lipoatrophy, severe osteoporosis, and marked osteolysis. The atrophic facial subcutaneous fat pad and the marked osteolysis of the maxilla and mandible result in a typical pseudosenile facial appearance with micrognatia, prominent subcutaneous venous patterning, a convex nasal ridge, and proptosis. Cognitive development is completely normal. Patients do not have cardiovascular dysfunction, atherosclerosis, or metabolic anomalies. Belongs to the BAF family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: mitotic nuclear envelope disassembly; mitotic nuclear envelope reassembly; nuclear transport; response to virus

Disease: Nestor-guillermo Progeria Syndrome