UNC13B blocking peptide

UNC13B Antibody (N-term) Blocking Peptide

• Gene Names: UNC13B; UNC13; MUNC13; Unc13h2
• Synonyms: UNC13B; UNC13B Antibody (N-term) Blocking Peptide; Protein unc-13 homolog B; Munc13-2; munc13; UNC13; UNC13B blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of UNC13B. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1591

• Cellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Cell membrane. Cell junction, synapse Note: Localized to synapses. Translocated to the plasma membrane in response to phorbol ester binding (By similarity).
• Tissue Location: Expressed in kidney cortical epithelial cells and brain.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for UNC13B blocking peptide

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 160332304
• NCBI GeneID: 10497
• UniProt Accession #: O14795; Q2NKJ5; Q5VYM8
• Molecular Weight: 182,843 Da
• NCBI Official Full Name: Protein unc-13 homolog B
• NCBI Official Synonym Full Names: unc-13 homolog B
• NCBI Official Symbol: UNC13B
• NCBI Official Synonym Symbols: UNC13; MUNC13; Unc13h2
• NCBI Protein Information: protein unc-13 homolog B
• UniProt Protein Name: Protein unc-13 homolog B
• Protein Family: Protein
• UniProt Gene Name: UNC13B
• UniProt Synonym Gene Names: UNC13; munc13
• UniProt Entry Name: UN13B_HUMAN

NCBI Description

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]

Uniprot Description

UNC13B: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Belongs to the unc-13 family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm; cytosol; Golgi apparatus; plasma membrane; presynaptic active zone; terminal button

Molecular Function: protein binding; receptor activity; signal transducer activity

Biological Process: apoptosis; excretion; glutamate secretion; neurotransmitter secretion; signal transduction; synaptic transmission; synaptic vesicle priming