RACGAP1 Blocking Peptide | RACGAP1 (HGNC:9804) blocking peptide

RACGAP1 Antibody (N-term) Blocking peptide

• Gene Names: RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• Synonyms: RACGAP1; RACGAP1 Antibody (N-term) Blocking peptide; Rac GTPase-activating protein 1; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4; RACGAP1 (HGNC:9804) blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 632

• Cellular Location: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome Cleavage furrow. Midbody. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with RND2 in Golgi- derived proacrosomal vesicles and the acrosome (By similarity) During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase
• Tissue Location: Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RACGAP1 (HGNC:9804) blocking peptide

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.

NCBI and Uniprot Product Information

• NCBI GI #: 74762727
• NCBI GeneID: 29127
• UniProt Accession #: Q9H0H5; Q6PJ26; Q9NWN2; Q9P250; Q9P2W2
• Molecular Weight: 71,027 Da
• NCBI Official Full Name: Rac GTPase-activating protein 1
• NCBI Official Synonym Full Names: Rac GTPase activating protein 1
• NCBI Official Symbol: RACGAP1
• NCBI Official Synonym Symbols: CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• NCBI Protein Information: rac GTPase-activating protein 1
• UniProt Protein Name: Rac GTPase-activating protein 1
• UniProt Gene Name: RACGAP1
• UniProt Synonym Gene Names: MgcRacGAP; CYK4; HsCYK-4
• UniProt Entry Name: RGAP1_HUMAN

NCBI Description

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

Uniprot Description

MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: cleavage furrow; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; midbody; nucleoplasm; nucleus; spindle midzone

Molecular Function: alpha-tubulin binding; beta-tubulin binding; gamma-tubulin binding; GTPase activator activity; microtubule binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cytokinesis after mitosis; cytokinesis, contractile ring formation; embryonic development; microtubule-based movement; neuroblast proliferation; positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; regulation of small GTPase mediated signal transduction; retrograde vesicle-mediated transport, Golgi to ER; spermatogenesis; spindle midzone assembly involved in mitosis; sulfate transport