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Western Blot (WB) (All lanes : Anti-RAB1B Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: U-20S whole cell lysateLane 4: rat liver lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Mouse RAB1B Monoclonal Antibody | anti-RAB1B antibody

RAB1B Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Synonyms
RAB1B; Monoclonal Antibody; RAB1B Antibody; Ras-related protein Rab-1B; anti-RAB1B antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This RAB1B antibody is generated from a mouse immunized with a recombinant protein between 1-201 amino acids from human RAB1B.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
201
Applicable Applications for anti-RAB1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:4000
Subtitle
Purified Mouse Monoclonal Antibody (Mab)
Cellular Location
Cytoplasm. Membrane; Lipid-anchor; Cytoplasmic side. Preautophagosomal structure membrane; Lipid-anchor; Cytoplasmic side. Note=Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm (By similarity).
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RAB1B Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: U-20S whole cell lysateLane 4: rat liver lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RAB1B Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: U-20S whole cell lysateLane 4: rat liver lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RAB1B antibody
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum.
Product Categories/Family for anti-RAB1B antibody
References
Zhao Y.,et al.Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases. Wiemann S.,et al.Genome Res. 11:422-435 (2001). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Bienvenut W.V.,et al.Submitted (JUN-2005) to UniProtKB. Wilson A.L.,et al.Biochem. J. 318:1007-1014 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22171 Da
NCBI Official Full Name
ras-related protein Rab-1B
NCBI Official Synonym Full Names
RAB1B, member RAS oncogene family
NCBI Official Symbol
RAB1B
NCBI Protein Information
ras-related protein Rab-1B
UniProt Protein Name
Ras-related protein Rab-1B
Protein Family
UniProt Gene Name
RAB1B
UniProt Entry Name
RAB1B_HUMAN

NCBI Description

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]

Uniprot Description

RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane; mitochondrion; pre-autophagosomal structure membrane; transport vesicle

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: autophagy; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; intracellular protein transport; metabolic process; nucleocytoplasmic transport; retrograde vesicle-mediated transport, Golgi to ER; small GTPase mediated signal transduction; virus assembly

Research Articles on RAB1B

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Product Notes

The RAB1B rab1b (Catalog #AAA9217041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:4000. Researchers should empirically determine the suitability of the RAB1B rab1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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