Rabbit anti-Mouse, Rat Syk Polyclonal Antibody | anti-Syk antibody

Mouse Syk Antibody (Center)

Cat. Num. AAA9215782

• Gene Names: Syk; Sykb
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Synonyms: Syk; Polyclonal Antibody; Mouse Syk Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Tyrosine-protein kinase SYK; Spleen tyrosine kinase; ptk72; Sykb; anti-Syk antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Mouse Syk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 318-352 amino acids from the Central region of Mouse Syk.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 629

• Applicable Applications for anti-Syk antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Syk Antibody (Center) at 1:2000 dilutionLane 1: mouse spleen lysatesLane 2: rat skeletal muscle lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 71 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Syk antibody

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine- phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T- cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen.

Product Categories/Family for anti-Syk antibody

Cardiovascular; Immunology; Signal Transduction

References

Turner M.,et al.Nature 378:298-302(1995). Flueck M.,et al.Biochem. Biophys. Res. Commun. 213:273-281(1995). Richards J.D.,et al.Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases. Kurosaki T.,et al.J. Exp. Med. 179:1725-1729(1994). Rowley R.B.,et al.J. Biol. Chem. 270:11590-11594(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 312176429
• NCBI GeneID: 20963
• NCBI Accession #: NP_001185906.1
• NCBI GenBank Nucleotide #: NM_001198977.1
• UniProt Accession #: P48025
• Molecular Weight: 71376
• NCBI Official Full Name: tyrosine-protein kinase SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Syk
• NCBI Official Synonym Symbols: Sykb
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: ptk72; Sykb
• UniProt Entry Name: KSYK_MOUSE

NCBI Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; transcription factor import into nucleus; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; activation of JNK activity; serotonin secretion; B cell receptor signaling pathway; leukocyte adhesion; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; angiogenesis; positive regulation of gamma-delta T cell differentiation; cell differentiation; integrin-mediated signaling pathway; neutrophil chemotaxis; cell migration; adaptive immune response; regulation of immune response; regulation of superoxide release; immune system process; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; neutrophil activation during immune response; leukocyte activation during immune response; serotonin secretion by platelet; macrophage activation during immune response; defense response to bacterium; blood vessel morphogenesis; innate immune response; positive regulation of B cell differentiation; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Cellular Component: B cell receptor complex; cytoplasm; cytoplasmic vesicle; extrinsic to internal side of plasma membrane; membrane; nucleus; plasma membrane; protein complex; T cell receptor complex

Molecular Function: ATP binding; integrin binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein domain specific binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein-tyrosine kinase activity; receptor binding; receptor signaling protein tyrosine kinase activity; transferase activity

Biological Process: activation of JNK activity; activation of MAPK activity; adaptive immune response; angiogenesis; B cell receptor signaling pathway; beta selection; blood vessel morphogenesis; cell surface receptor linked signal transduction; defense response to bacterium; enzyme linked receptor protein signaling pathway; G-protein coupled receptor protein signaling pathway; immune system process; innate immune response; integrin-mediated signaling pathway; leukocyte activation during immune response; leukocyte adhesion; leukotriene biosynthetic process; lymph vessel development; macrophage activation during immune response; neutrophil activation during immune response; neutrophil chemotaxis; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of alpha-beta T cell differentiation; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell differentiation; positive regulation of bone resorption; positive regulation of calcium-mediated signaling; positive regulation of cell adhesion mediated by integrin; positive regulation of cytokine secretion; positive regulation of gamma-delta T cell differentiation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interferon type I production; positive regulation of interleukin-3 biosynthetic process; positive regulation of mast cell degranulation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of receptor internalization; protein amino acid autophosphorylation; protein amino acid phosphorylation; receptor internalization; regulation of immune response; regulation of neutrophil degranulation; regulation of phagocytosis; regulation of superoxide release; regulation of transcription factor activity; serotonin secretion; serotonin secretion by platelet; transcription factor import into nucleus; transmembrane receptor protein tyrosine kinase signaling pathway