Western Blot (WB)
(All lanes : Anti-Ptk7 Antibody (C-term) at1:1000-1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: mouse lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Ptk7 Polyclonal Antibody | anti-Ptk7 antibody
Mouse Ptk7 Antibody (C-term)
WB ~~ 1:2000
WB ~~ 1:1000
Western Blot (WB)
(All lanes : Anti-Ptk7 Antibody (C-term) at1:1000-1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: mouse lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Ptk7 Antibody (C-term) at1:1000-1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: mouse lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Ptk7 Antibody (C-term) at 1:2000 dilution + NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Ptk7 Antibody (C-term) at 1:2000 dilution + NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Ptk7 Antibody (C-term) at 1:1000 dilution + C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Ptk7 Antibody (C-term) at 1:1000 dilution + C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.10.1; Membrane protein, integral; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; CCK4 family
Cellular Component: focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intercellular junction; cell junction
Molecular Function: protein-tyrosine kinase activity; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity
Biological Process: cell migration; Wnt receptor signaling pathway; wound healing; convergent extension; actin cytoskeleton reorganization; neural tube closure; establishment of epithelial cell polarity; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; cell adhesion; protein amino acid phosphorylation
Uniprot Description
CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; CCK4 family
Cellular Component: cell junction; focal adhesion; integral to membrane; intercellular junction; membrane; plasma membrane
Molecular Function: ATP binding; kinase activity; protein kinase activity; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: actin cytoskeleton reorganization; cell adhesion; cell migration; convergent extension; establishment of epithelial cell polarity; establishment of planar polarity; heart development; neural tube closure; protein amino acid phosphorylation; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; wound healing
Research Articles on Ptk7
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Product Notes
The Ptk7 ptk7 (Catalog #AAA9215627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Ptk7 Antibody (C-term) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ptk7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:1000-1:2000 WB ~~ 1:2000 WB ~~ 1:1000. Researchers should empirically determine the suitability of the Ptk7 ptk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ptk7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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