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Western Blot (WB) (CTF1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CTF1 antibody detected the CTF1 protein (arrow).)

Rabbit anti-Human CTF1 Polyclonal Antibody | anti-CTF1 antibody

CTF1 Antibody (C-term)

Gene Names
CTF1; CT1; CT-1
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CTF1; Polyclonal Antibody; CTF1 Antibody (C-term); Cardiotrophin-1; CT-1; anti-CTF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CTF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the C-terminal region of human CTF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
166-194
Sequence Length
200
Applicable Applications for anti-CTF1 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(CTF1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CTF1 antibody detected the CTF1 protein (arrow).)

Western Blot (WB) (CTF1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CTF1 antibody detected the CTF1 protein (arrow).)

Flow Cytometry (FC/FACS)

(CTF1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (CTF1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(CTF1 Antibody (C-term) (MBS9215186)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (CTF1 Antibody (C-term) (MBS9215186)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CTF1 antibody
The protein encoded by this gene is a secreted cytokine
that induces cardiac myocyte hypertrophy in vitro. It has been
shown to bind and activate the ILST/gp130 receoptor. Two transcript
variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-CTF1 antibody
References
Robador, P.A., et al. J. Hypertens. (2010) In press :
Bailey, S.D., et al. Diabetes Care (2010) In press :
Limongelli, G., et al. Cytokine 50(3):245-247(2010)
Zimmerman, R.S., et al. Genet. Med. 12(5):268-278(2010)
Fritzenwanger, M., et al. Mediators Inflamm. 2009, 489802 (2009) :

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21227
NCBI Official Full Name
cardiotrophin-1 isoform 2
NCBI Official Synonym Full Names
cardiotrophin 1
NCBI Official Symbol
CTF1
NCBI Official Synonym Symbols
CT1; CT-1
NCBI Protein Information
cardiotrophin-1
UniProt Protein Name
Cardiotrophin-1
Protein Family
UniProt Gene Name
CTF1
UniProt Synonym Gene Names
CT-1
UniProt Entry Name
CTF1_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

CTF1: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: leukemia inhibitory factor receptor binding; cytokine activity

Biological Process: nervous system development; cell proliferation; muscle development; cell-cell signaling; leukemia inhibitory factor signaling pathway; positive regulation of cell proliferation; neuron development

Research Articles on CTF1

Similar Products

Product Notes

The CTF1 ctf1 (Catalog #AAA9215186) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 166-194. The CTF1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CTF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CTF1 ctf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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