Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.colon carcinoma section using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Rabbit anti-Human PCSK9 Polyclonal Antibody | anti-PCSK9 antibody
PCSK9 Antibody (C-term)
IHC-P~~1:100
FC~~1:10~50
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.colon carcinoma section using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.colon carcinoma section using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody (C-term). MBS9214204 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Immunohistochemistry (IHC)
(PCSK9 Antibody (C-term) (RB18880) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCSK9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PCSK9 Antibody (C-term) (RB18880) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCSK9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PCSK9 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PCSK9 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
McNutt,M.C., Kwon,H.J. J. Biol. Chem. 284 (16), 10561-10570 (2009)
Shioji,K., Mannami,T. J. Hum. Genet. 49 (2), 109-114 (2004)
Abifadel,M., Varret,M. Nat. Genet. 34 (2), 154-156 (2003)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Secreted; Protease; EC 3.4.21.-; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p32.3
Cellular Component: Golgi apparatus; extracellular space; extrinsic to external side of plasma membrane; cell surface; rough endoplasmic reticulum; endoplasmic reticulum; lysosome; perinuclear region of cytoplasm; early endosome; cytoplasm; late endosome; plasma membrane; ER to Golgi transport vesicle
Molecular Function: sodium channel inhibitor activity; very-low-density lipoprotein binding; protein binding; protein self-association; low-density lipoprotein receptor binding; serine-type endopeptidase activity; low-density lipoprotein binding; apolipoprotein binding; apolipoprotein receptor binding
Biological Process: cholesterol metabolic process; lysosomal transport; apoptosis; positive regulation of receptor internalization; lipoprotein metabolic process; regulation of low-density lipoprotein receptor catabolic process; liver development; proteolysis; cellular response to starvation; neuron differentiation; protein autoprocessing; cholesterol homeostasis; triacylglycerol metabolic process; cellular response to insulin stimulus; neurogenesis; positive regulation of neuron apoptosis; phospholipid metabolic process; regulation of neuron apoptosis; negative regulation of receptor recycling; low-density lipoprotein receptor catabolic process; regulation of receptor activity; kidney development
Disease: Hypercholesterolemia, Autosomal Dominant, 3
Research Articles on PCSK9
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Product Notes
The PCSK9 pcsk9 (Catalog #AAA9214204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 479-508. The PCSK9 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCSK9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-paraffin (IHC-p), Flow Cytometry (FC), Western Blot (WB). WB~~1:1000 IHC-P~~1:100 FC~~1:10~50. Researchers should empirically determine the suitability of the PCSK9 pcsk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCSK9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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