Testing Data
(All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit KLF9 Polyclonal Antibody | anti-KLF9 antibody
KLF9 Antibody (N-term)
IF~~1;10~50
Testing Data
(All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(Anti-KLF9 Antibody (N-term) at 1:2000 dilution+ U-87 MG whole cell lysate Lysates/proteins at20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(Anti-KLF9 Antibody (N-term) at 1:2000 dilution+ U-87 MG whole cell lysate Lysates/proteins at20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
( KLF9 Antibody (N-term)western blot analysis in WiDr cell line lysates(35ug/lane).This demonstrates the KLF9antibody detected the KLF9 protein (arrow).)
Testing Data (TD)
( KLF9 Antibody (N-term)western blot analysis in WiDr cell line lysates(35ug/lane).This demonstrates the KLF9antibody detected the KLF9 protein (arrow).)
Testing Data (TD)
( Fluorescent confocal image of U251 cellstained with KLF9 Antibody(N-term).U251 cells were fixedwith 4% PFA (20 min), permeabilized withTriton X-100 (0.1%, 10 min), then incubatedwith KLF9 primary antibody (1:25, 1 h at 37?).For secondary antibody, Alexa Fluor® 488conjugated donkey anti-rabbit antibody (green)was used (1:400, 50 min at 37?).Cytoplasmicactin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 hat 37?). Nuclei were counterstained with DAPI(blue) (10 µg/ml, 10 min). KLF9immunoreactivity is localized to vesicles andNucleus significantly.)
Testing Data (TD)
( Fluorescent confocal image of U251 cellstained with KLF9 Antibody(N-term).U251 cells were fixedwith 4% PFA (20 min), permeabilized withTriton X-100 (0.1%, 10 min), then incubatedwith KLF9 primary antibody (1:25, 1 h at 37?).For secondary antibody, Alexa Fluor® 488conjugated donkey anti-rabbit antibody (green)was used (1:400, 50 min at 37?).Cytoplasmicactin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 hat 37?). Nuclei were counterstained with DAPI(blue) (10 µg/ml, 10 min). KLF9immunoreactivity is localized to vesicles andNucleus significantly.)
that binds to GC box elements located in the promoter. Binding of
the encoded protein to a single GC box inhibits mRNA expression
while binding to tandemly repeated GC box elements activates
transcription.
Pabona, J.M., et al. Endocrinology 151(7):3396-3406(2010)
Gamper, I., et al. Exp. Hematol. 37(5):539-548(2009)
Kang, L., et al. Pathol. Int. 58(6):334-338(2008)
Simmen, F.A., et al. Reprod. Biol. Endocrinol. 6, 41 (2008) :
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. [provided by RefSeq, Jul 2008]
Uniprot Description
KLF9: Transcription factor that binds to GC box promoter elements. Selectively activates mRNA synthesis from genes containing tandem repeats of GC boxes but represses genes with a single GC box. Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 9q13
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus
Molecular Function: DNA binding; metal ion binding; transcription factor activity
Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; progesterone receptor signaling pathway; transcription, DNA-dependent; embryo implantation
Research Articles on KLF9
Similar Products
Product Notes
The KLF9 klf9 (Catalog #AAA9212765) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 29-57. The KLF9 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's KLF9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000 IF~~1;10~50. Researchers should empirically determine the suitability of the KLF9 klf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
