Western Blot (WB)
(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)
Rabbit TGIF1 Polyclonal Antibody | anti-TGIF1 antibody
TGIF1 Antibody (Center L223)
Western Blot (WB)
(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)
Western Blot (WB)
(Western blot analysis of TGIF1 (arrow) using rabbit polyclonal TGIF1 Antibody (Center L223). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGIF1 gene.)
Western Blot (WB)
(TGIF1 Antibody (Center L223) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).)
Western Blot (WB)
(TGIF1 Antibody (Center L223) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TGIF1 antibody detected the TGIF1 protein (arrow).)
three-amino acid loop extension (TALE) superclass of atypical
homeodomains. TALE homeobox proteins are highly conserved
transcription regulators. This particular homeodomain binds to a
previously characterized retinoid X receptor responsive element
from the cellular retinol-binding protein II promoter. In addition
to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha
transcription activation of the retinoic acid responsive element,
the protein is an active transcriptional co-repressor of SMAD2 and
may participate in the transmission of nuclear signals during
development and in the adult. Mutations in this gene are associated
with holoprosencephaly type 4, which is a structural anomaly of the
brain. Alternative splicing has been observed at this locus and
eight variants, encoding four distinct isoforms, are described.
Paulussen, A.D., et al. Eur. J. Hum. Genet. 18(9):999-1005(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Demange, C., et al. Mol. Cell 36(6):1073-1085(2009)
Hamid, R., et al. Mol Oncol 3 (5-6), 451-463 (2009) :
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2013]
Uniprot Description
TGIF: Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities. Defects in TGIF1 are the cause of holoprosencephaly type 4 (HPE4). Holoprosencephaly (HPE) is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Belongs to the TALE/TGIF homeobox family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 18p11.3
Cellular Component: nucleoplasm
Molecular Function: protein binding; chromatin binding; transcription factor activity; transcription corepressor activity
Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; dorsal/ventral pattern formation; retina development in camera-type eye; transforming growth factor beta receptor signaling pathway; regulation of gastrulation; neural tube closure; gene expression; positive regulation of neuron differentiation; determination of left/right symmetry
Disease: Holoprosencephaly 4
Research Articles on TGIF1
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Product Notes
The TGIF1 tgif1 (Catalog #AAA9212637) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 208-237. The TGIF1 Antibody (Center L223) reacts with Human, mouse (Predicted Reactivity: Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's TGIF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the TGIF1 tgif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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