Western Blot (WB)
(Anti-ENT1 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit ENT1 Polyclonal Antibody | anti-SLC29A1 antibody
ENT1 Antibody
Western Blot (WB)
(Anti-ENT1 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-ENT1 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Graham K.A.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Lum P.Y.,et al.Cancer Chemother. Pharmacol. 45:273-278(2000).
Sankar N.,et al.Nucleic Acids Res. 30:4339-4350(2002).
Mangravite L.M.,et al.Am. J. Physiol. 284:F902-F910(2003).
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: membrane; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane
Molecular Function: nucleoside transmembrane transporter activity
Biological Process: lactation; uridine transport; nucleoside transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; regulation of excitatory postsynaptic membrane potential; sleep; transmembrane transport
Research Articles on SLC29A1
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Product Notes
The SLC29A1 slc29a1 (Catalog #AAA9212462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-60. The ENT1 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's ENT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~ 1:1000. Researchers should empirically determine the suitability of the SLC29A1 slc29a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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