Western Blot (WB)
(MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).)
Rabbit anti-Human MIA40 Polyclonal Antibody | anti-CHCHD4 antibody
MIA40 Antibody (C-term)
Western Blot (WB)
(MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).)
Western Blot (WB)
(MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).)
Immunohistochemistry (IHC)
(MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
protein family whose members share 6 highly conserved cysteine
residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus
(Hofmann et al., 2005 [PubMed 16185709]).
Chacinska, A., et al. J. Biol. Chem. 283(44):29723-29729(2008)
Terziyska, N., et al. FEBS Lett. 581(6):1098-1102(2007)
Hofmann, S., et al. J. Mol. Biol. 353(3):517-528(2005)
NCBI and Uniprot Product Information
NCBI Description
CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]).[supplied by OMIM, Mar 2008]
Uniprot Description
CHCHD4: Required for the import and folding of small cysteine- containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 3p25.1
Cellular Component: mitochondrion; mitochondrial intermembrane space
Molecular Function: protein disulfide oxidoreductase activity
Biological Process: 'de novo' posttranslational protein folding; protein maturation via protein folding; cellular protein metabolic process; protein import into mitochondrial intermembrane space; protein targeting to mitochondrion
Research Articles on CHCHD4
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Product Notes
The CHCHD4 chchd4 (Catalog #AAA9212120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 101-129. The MIA40 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIA40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the CHCHD4 chchd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIA40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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