Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H.kidney section using PDCL Antibody (N-term). MBS9211356 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Rabbit PDCL Polyclonal Antibody | anti-PDCL antibody

PDCL Antibody (N-term)

Gene Names
PDCL; PhLP
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PDCL; Polyclonal Antibody; PDCL Antibody (N-term); Phosducin-like protein; PHLP; PHLOP1; PhLP1; anti-PDCL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PDCL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PDCL.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
301
Applicable Applications for anti-PDCL antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC~~1:25
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H.kidney section using PDCL Antibody (N-term). MBS9211356 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H.kidney section using PDCL Antibody (N-term). MBS9211356 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysates from Hela, Y79 cell line (from left to right), using PDCL Antibody (N-term). MBS9211356 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from Hela, Y79 cell line (from left to right), using PDCL Antibody (N-term). MBS9211356 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-PDCL antibody
Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.
Product Categories/Family for anti-PDCL antibody
References
Lazarov M.E.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34282
NCBI Official Full Name
phosducin-like protein
NCBI Official Synonym Full Names
phosducin-like
NCBI Official Symbol
PDCL
NCBI Official Synonym Symbols
PhLP
NCBI Protein Information
phosducin-like protein
UniProt Protein Name
Phosducin-like protein
Protein Family
UniProt Gene Name
PDCL
UniProt Synonym Gene Names
PHLOP1; PhLP1; PHLP
UniProt Entry Name
PHLP_HUMAN

NCBI Description

Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PhLP: a member of the phosducin family of G-protein beta-gamma regulators. Shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. Two splice variants have been described.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 9q12-q13

Cellular Component: cytoplasm

Molecular Function: protein binding; receptor signaling protein activity; protein complex binding

Biological Process: protein folding; visual perception; signal transduction; regulation of G-protein coupled receptor protein signaling pathway

Research Articles on PDCL

Similar Products

Product Notes

The PDCL pdcl (Catalog #AAA9211356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDCL Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PDCL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the PDCL pdcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.