Western Blot (WB)
(Western blot analysis of PLCZ1 Antibody (N-term) in CEM, 293 cell line and mouse heart,spleen tissue lysates (35ug/lane). PLCZ1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse PLCZ1 Polyclonal Antibody | anti-PLCZ1 antibody
PLCZ1 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of PLCZ1 Antibody (N-term) in CEM, 293 cell line and mouse heart,spleen tissue lysates (35ug/lane). PLCZ1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PLCZ1 Antibody (N-term) in CEM, 293 cell line and mouse heart,spleen tissue lysates (35ug/lane). PLCZ1 (arrow) was detected using the purified Pab.)
Testing Data(TD)
( Anti-PLCZ1 Antibody (N-term) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Testing Data(TD)
( Anti-PLCZ1 Antibody (N-term) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Ito, M., et al. Biol. Reprod. 78(6):1081-1090(2008)
Yu, Y., et al. Hum. Reprod. 23(2):365-373(2008)
Yoshida, N., et al. Development 134(21):3941-3952(2007)
Zhu, J.X., et al. Cell Biol. Int. 31(5):521-525(2007)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
PLCZ1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase
Chromosomal Location of Human Ortholog: 12p12.3
Cellular Component: perinuclear region of cytoplasm; cytosol; nucleus
Molecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activity
Biological Process: inositol phosphate metabolic process; multicellular organismal development; calcium ion transport; egg activation; lipid catabolic process
Research Articles on PLCZ1
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Product Notes
The PLCZ1 plcz1 (Catalog #AAA9209842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 26-54. The PLCZ1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLCZ1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PLCZ1 plcz1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCZ1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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