Western Blot (WB)
(RICH2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RICH2 antibody detected the RICH2 protein (arrow).)
Rabbit RICH2 Polyclonal Antibody | anti-ARHGAP44 antibody
RICH2 Antibody (C-term)
Gene Names
ARHGAP44; RICH2; NPC-A-10
Reactivity
Human (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RICH2; Polyclonal Antibody; RICH2 Antibody (C-term); Rho GTPase-activating protein 44; NPC-A-10; Rho-type GTPase-activating protein RICH2; RhoGAP interacting with CIP4 homologs protein 2; RICH-2; ARHGAP44; KIAA0672; anti-ARHGAP44 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RICH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-811 amino acids from the C-terminal region of human RICH2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
784-811
Sequence Length
812
Applicable Applications for anti-ARHGAP44 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(RICH2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RICH2 antibody detected the RICH2 protein (arrow).)
Western Blot (WB)
(RICH2 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RICH2 antibody detected the RICH2 protein (arrow).)
Immunohistochemistry (IHC)
(RICH2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RICH2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RICH2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RICH2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RICH2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RICH2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ARHGAP44 antibody
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Acts as a GTPase activitor in vitro for CDC42 and RAC1.
Product Categories/Family for anti-ARHGAP44 antibody
References
Rollason, R., et al. J. Cell Biol. 184(5):721-736(2009)
Richnau, N., et al. J. Biol. Chem. 276(37):35060-35070(2001)
Richnau, N., et al. J. Biol. Chem. 276(37):35060-35070(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89247
NCBI Official Full Name
rho GTPase-activating protein 44
NCBI Official Synonym Full Names
Rho GTPase activating protein 44
NCBI Official Symbol
ARHGAP44
NCBI Official Synonym Symbols
RICH2; NPC-A-10
NCBI Protein Information
rho GTPase-activating protein 44
UniProt Protein Name
Rho GTPase-activating protein 44
Protein Family
UniProt Gene Name
ARHGAP44
UniProt Synonym Gene Names
KIAA0672; RICH2; RICH-2
UniProt Entry Name
RHG44_HUMAN
Uniprot Description
RICH2: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Acts as a GTPase activitor in vitro for CDC42 and RAC1.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 17p12
Cellular Component: recycling endosome; dendritic spine; synapse; cell junction; cytosol
Molecular Function: protein binding; phospholipid binding; GTPase activator activity
Biological Process: regulation of small GTPase mediated signal transduction; exocytosis; small GTPase mediated signal transduction; positive regulation of GTPase activity
Research Articles on ARHGAP44
Similar Products
Product Notes
The ARHGAP44 arhgap44 (Catalog #AAA9207449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 784-811. The RICH2 Antibody (C-term) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RICH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ARHGAP44 arhgap44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RICH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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