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Western Blot (WB) (Western blot analysis of FPRL2 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). FPRL2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human FPRL2 Polyclonal Antibody | anti-FPR3 antibody

FPRL2 Antibody (Center)

Gene Names
FPR3; FMLPY; FPRH1; FPRH2; FPRL2; RMLP-R-I; FML2_HUMAN
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FPRL2; Polyclonal Antibody; FPRL2 Antibody (Center); N-formyl peptide receptor 3; FMLP-related receptor II; FMLP-R-II; Formyl peptide receptor-like 2; FPR3; FPRH1; anti-FPR3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FPRL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-333 amino acids from the Central region of human FPRL2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
307-333
Sequence Length
353
Applicable Applications for anti-FPR3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of FPRL2 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). FPRL2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of FPRL2 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). FPRL2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with FPRL2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with FPRL2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FPRL2 Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (FPRL2 Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-FPR3 antibody
Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.
Product Categories/Family for anti-FPR3 antibody
References
Yang,D., et.al., J. Leukoc. Biol. 72 (3), 598-607 (2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39965
NCBI Official Full Name
N-formyl peptide receptor 3
NCBI Official Synonym Full Names
formyl peptide receptor 3
NCBI Official Symbol
FPR3
NCBI Official Synonym Symbols
FMLPY; FPRH1; FPRH2; FPRL2; RMLP-R-I; FML2_HUMAN
NCBI Protein Information
N-formyl peptide receptor 3
UniProt Protein Name
N-formyl peptide receptor 3
Protein Family
UniProt Gene Name
FPR3
UniProt Synonym Gene Names
FPRH1; FPRL2; FMLP-R-II
UniProt Entry Name
FPR3_HUMAN

Uniprot Description

FPR3: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: N-formyl peptide receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell motility; chemotaxis; signal transduction

Research Articles on FPR3

Similar Products

Product Notes

The FPR3 fpr3 (Catalog #AAA9204990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 307-333. The FPRL2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FPRL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the FPR3 fpr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FPRL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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