Rabbit anti-Human, mouse Rad9a Polyclonal Antibody | anti-Rad9a antibody

Mouse Rad9a Antibody (C-term)

Cat. Num. AAA9204164

• Gene Names: Rad9a; Rad9
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Rad9a; Polyclonal Antibody; Mouse Rad9a Antibody (C-term); Cell cycle checkpoint control protein RAD9A; mRAD9; DNA repair exonuclease rad9 homolog A; Rad9-like protein; Rad9; anti-Rad9a antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Rad9a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 279-313 amino acids from the C-terminal region of Mouse Rad9a.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 389

• Applicable Applications for anti-Rad9a antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Rad9a Antibody (C-term) at 1:2000 dilutionLane 1: mouse spleen lysatesLane 2: mouse heart lysatesLane 3: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-Rad9a Antibody (C-term)at 1:1000 dilution + mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Rad9a antibody

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).

References

Hang H.,et al.J. Cell. Physiol. 177:241-247(1998).
Carninci P.,et al.Science 309:1559-1563(2005).
Park Y.-G.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
Ishii H.,et al.Proc. Natl. Acad. Sci. U.S.A. 102:9655-9660(2005).
Sweet S.M.,et al.Mol. Cell. Proteomics 8:904-912(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6755282
• NCBI GeneID: 19367
• NCBI Accession #: NP_035367.1
• NCBI GenBank Nucleotide #: NM_011237.2
• UniProt Accession #: Q9Z0F6; Q8QZZ3
• Molecular Weight: 42059
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A
• NCBI Official Synonym Full Names: RAD9 homolog A
• NCBI Official Symbol: Rad9a
• NCBI Official Synonym Symbols: Rad9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: Cell cycle checkpoint control protein
• UniProt Gene Name: Rad9a
• UniProt Synonym Gene Names: Rad9; mRAD9
• UniProt Entry Name: RAD9A_MOUSE

Uniprot Description

Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: DNA replication; EC 3.1.11.2; Cell cycle regulation; Apoptosis; Deoxyribonuclease

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; nuclease activity; hydrolase activity; histone deacetylase binding; exonuclease activity; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; protein kinase binding; SH3 domain binding

Biological Process: response to radiation; intra-S DNA damage checkpoint; DNA damage checkpoint; DNA repair; response to DNA damage stimulus; cell cycle checkpoint