Western Blot (WB)
(Western blot analysis of lysates from U-87 MG,SH-SY5Y cell line (from left to right),using NSE Antibody(C0280). C0280 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Rabbit NSE Polyclonal Antibody | anti-ENO2 antibody
NSE Antibody
Western Blot (WB)
(Western blot analysis of lysates from U-87 MG,SH-SY5Y cell line (from left to right),using NSE Antibody(C0280). C0280 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from U-87 MG,SH-SY5Y cell line (from left to right),using NSE Antibody(C0280). C0280 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells (Lane 1), colo cells(Lane 2)?HepG2 cells(Lane 3) and 293 cells (Lane 4), using NSE Antibody. The lane on the left is treated with synthesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells (Lane 1), colo cells(Lane 2)?HepG2 cells(Lane 3) and 293 cells (Lane 4), using NSE Antibody. The lane on the left is treated with synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using NSE antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using NSE antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using NSE antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using NSE antibody.)
van Obberghen E.,et al.J. Neurosci. Res. 19:450-456(1988).
Oliva D.,et al.Gene 79:355-360(1989).
Oliva D.,et al.Genomics 10:157-165(1991).
Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: extracellular space; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane; perikaryon; cytosol
Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity
Biological Process: glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis
Research Articles on ENO2
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Product Notes
The ENO2 eno2 (Catalog #AAA9203509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 392-425. The NSE Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's NSE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). WB~~ 1:1000. Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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