Western Blot (WB)
(TAF1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TAF1 antibody detected the TAF1 protein (arrow).)
Rabbit anti-Mouse TAF1 Polyclonal Antibody | anti-TAF1 antibody
TAF1 Antibody (C-term)
Western Blot (WB)
(TAF1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TAF1 antibody detected the TAF1 protein (arrow).)
Western Blot (WB)
(TAF1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TAF1 antibody detected the TAF1 protein (arrow).)
the activities of more than 70 polypeptides. The protein that
coordinates these activities is the basal transcription factor
TFIID, which binds to the core promoter to position the polymerase
properly, serves as the scaffold for assembly of the remainder of
the transcription complex, and acts as a channel for regulatory
signals. TFIID is composed of the TATA-binding protein (TBP) and a
group of evolutionarily conserved proteins known as TBP-associated
factors or TAFs. TAFs may participate in basal transcription, serve
as coactivators, function in promoter recognition or modify general
transcription factors (GTFs) to facilitate complex assembly and
transcription initiation. This gene encodes the largest subunit of
TFIID. This subunit binds to core promoter sequences encompassing
the transcription start site. It also binds to activators and other
transcriptional regulators, and these interactions affect the rate
of transcription initiation. This subunit contains two independent
protein kinase domains at the N and C-terminals, but also possesses
acetyltransferase activity and can act as a
ubiquitin-activating/conjugating enzyme. This gene is part of a
complex transcriptional unit (TAF1/DYT3), wherein some products
share exons with TAF1 as well as additional exons
downstream.
Allende-Vega, N., et al. Mol. Cell. Biochem. 316 (1-2), 99-106 (2008) :
Centeno, F., et al. Intervirology 51(2):137-143(2008)
Olejnik-Schmidt, A.K., et al. Arch. Virol. 153(5):983-990(2008)
Li, A.G., et al. Mol. Cell 28(3):408-421(2007)
NCBI and Uniprot Product Information
NCBI Description
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]
Uniprot Description
TAF1: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3); also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Belongs to the TAF1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; DNA-binding; EC 2.3.1.48; Protein kinase, atypical; EC 2.7.11.1; Transcription, coactivator/corepressor; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; TAF1 family
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: nucleoplasm; transcription factor TFIID complex
Molecular Function: protein serine/threonine kinase activity; protein binding; histone acetyltransferase activity; TATA-binding protein binding; sequence-specific DNA binding; p53 binding; transcription coactivator activity; transcription factor binding; ATP binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; protein amino acid autophosphorylation; transcription initiation; peptidyl-threonine phosphorylation; transcriptional preinitiation complex assembly; cell cycle; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; histone acetylation; response to DNA damage stimulus
Disease: Dystonia 3, Torsion, X-linked
Research Articles on TAF1
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Product Notes
The TAF1 taf1 (Catalog #AAA9202604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1863-1893. The TAF1 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the TAF1 taf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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