Western Blot (WB)
(DGKZ Antibody (D1004) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).)
Rabbit anti-Human DGKZ Polyclonal Antibody | anti-DGKZ antibody
DGKZ Antibody (C-term)
Western Blot (WB)
(DGKZ Antibody (D1004) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).)
Western Blot (WB)
(DGKZ Antibody (D1004) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).)
Western Blot (WB)
(Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunofluorescence (IF)
(SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.)
Immunofluorescence (IF)
(SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.)
signaling cascade and signal transduction.
Topham, M.K., et al., Nature 394(6694):697-700 (1998).
Ding, L., et al., Proc. Natl. Acad. Sci. U.S.A. 94(11):5519-5524 (1997).
Bunting, M., et al., J. Biol. Chem. 271(17):10230-10236 (1996).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010]
Uniprot Description
DGKZ: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. Belongs to the eukaryotic diacylglycerol kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Kinase, lipid
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nucleoplasm; lamellipodium; cytoplasm; plasma membrane; nucleus
Molecular Function: enzyme inhibitor activity; protein C-terminus binding; lipid kinase activity; protein binding; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity
Biological Process: G1 DNA damage checkpoint; platelet activation; cell migration; protein kinase C activation; negative regulation of catalytic activity; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; lipid phosphorylation; blood coagulation; phosphorylation
Research Articles on DGKZ
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Product Notes
The DGKZ dgkz (Catalog #AAA9202267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 989-1019. The DGKZ Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DGKZ can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the DGKZ dgkz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKZ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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