Rabbit ANKH Polyclonal Antibody | anti-ANKH antibody

ANKH Antibody (C-term)

Cat. Num. AAA9201847

• Gene Names: ANKH; ANK; CMDJ; HANK; MANK; CCAL2; CPPDD
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ANKH; Polyclonal Antibody; ANKH Antibody (C-term); Progressive ankylosis protein homolog; ANK; KIAA1581; anti-ANKH antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 464-492
• Sequence Length: 492

• Applicable Applications for anti-ANKH antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; IHC-p~~1:10~50; FC~~1:10~50
• Function: Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter.
• Cellular Location: Membrane; Multi-pass membrane protein
• Tissue Location: Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ANKH Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ANKH Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC)

(ANKH Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ANKH antibody

ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.

Product Categories/Family for anti-ANKH antibody

Signal Transduction

References

Wang, J., et al. J. Rheumatol. 36(6):1265-1272(2009)
Ho, A.M., et al. Science 289(5477):265-270(2000)
Rojas, K., et al. Genomics 62(2):177-183(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 16905507
• NCBI GeneID: 56172
• NCBI Accession #: NP_473368.1
• NCBI GenBank Nucleotide #: NM_054027.4
• UniProt Accession #: Q9HCJ1; Q9NQW2; B2RCA7; B3KMG4; D3DTD4
• NCBI Official Full Name: progressive ankylosis protein homolog
• NCBI Official Synonym Full Names: ANKH inorganic pyrophosphate transport regulator
• NCBI Official Symbol: ANKH
• NCBI Official Synonym Symbols: ANK; CMDJ; HANK; MANK; CCAL2; CPPDD
• NCBI Protein Information: progressive ankylosis protein homolog
• UniProt Protein Name: Progressive ankylosis protein homolog
• Protein Family: Progressive ankylosis protein
• UniProt Gene Name: ANKH
• UniProt Synonym Gene Names: KIAA1581; ANK
• UniProt Entry Name: ANKH_HUMAN

NCBI Description

This gene encodes a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals. Mutations in this gene have been associated with autosomal dominant craniometaphyseal dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

ANKH: Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter. Defects in ANKH are the cause of chondrocalcinosis 2 (CCAL2). Chondrocalcinosis is a common cause of joint pain and arthritis caused by calcium deposition in articular cartilage and the presence of calcium hypophosphate crystals in synovial fluid, cartilage and periarticular soft tissue. CCAL2 inheritance is autosomal dominant. Defects in ANKH are the cause of craniometaphyseal dysplasia Jackson type (CMDJ). CMDJ is a rare autosomal dominant skeletal disorder characterized by abnormal bone formation and mineralization in membranous as well as endochondral bones. Progressive thickening of the bones can cause narrowing of cranial foramina and can lead to severe visual and neurological impairment, such as facial palsy and deafness. Belongs to the ANKH family.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.1

Cellular Component: outer membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: phosphate transmembrane transporter activity; inorganic diphosphate transmembrane transporter activity; inorganic phosphate transmembrane transporter activity

Biological Process: locomotory behavior; skeletal development; regulation of bone mineralization; inorganic diphosphate transport

Disease: Craniometaphyseal Dysplasia, Autosomal Dominant; Chondrocalcinosis 2