Western Blot (WB)
(HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).)
Rabbit anti-Human HDAC4 Polyclonal Antibody | anti-HDAC4 antibody
HDAC4 Antibody (C-term)
Western Blot (WB)
(HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).)
Western Blot (WB)
(HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).)
Immunohistochemistry (IHC)
(HDAC4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HDAC4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.)
regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and
affects transcription factor access to DNA. The protein encoded by
this gene belongs to class II of the histone deacetylase/acuc/apha
family. It possesses histone deacetylase activity and represses
transcription when tethered to a promoter. This protein does not
bind DNA directly, but through transcription factors MEF2C and
MEF2D. It seems to interact in a multiprotein complex with RbAp48
and HDAC3.
Williams, S.R., et al. Am. J. Hum. Genet. 87(2):219-228(2010)
Kim, T., et al. Psychiatry Res 178(2):266-269(2010)
Pan, L., et al. Cell. Mol. Immunol. 7(3):221-226(2010)
Smith, J.A., et al. Virol. J. 7, 237 (2010) :
NCBI and Uniprot Product Information
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Protein type: Hydrolase; Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; histone deacetylase complex; actomyosin; neuromuscular junction; nucleus; Z disc; cytosol; A band
Molecular Function: potassium ion binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription activator binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding; transcription corepressor activity; protein kinase binding
Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; response to denervation involved in regulation of muscle adaptation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; negative regulation of glycolysis; inflammatory response; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; regulation of protein binding; B cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Research Articles on HDAC4
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Product Notes
The HDAC4 hdac4 (Catalog #AAA9201247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1049-1077. The HDAC4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the HDAC4 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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