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Western Blot (WB) (AGPAT9 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).)

Rabbit AGPAT9 Polyclonal Antibody | anti-AGPAT9 antibody

AGPAT9 Antibody (Center)

Gene Names
AGPAT9; MAG1; GPAT3; AGPAT8; AGPAT 10; HMFN0839; LPAAT-theta
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AGPAT9; Polyclonal Antibody; AGPAT9 Antibody (Center); Glycerol-3-phosphate acyltransferase 3; GPAT-3; 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10; AGPAT 10; 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9; 1-AGP acyltransferase 9; 1-AGPAT 9; Acyl-CoA:glycerol-3-phosphate acyltransferase 3; hGPAT3; Lung cancer metastasis-associated protein 1; Lysophosphatidic acid acyltransferase theta; LPAAT-theta; MAG-1; GPAT3; MAG1; anti-AGPAT9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AGPAT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-222 amino acids from the Central region of human AGPAT9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
194-222
Sequence Length
434
Applicable Applications for anti-AGPAT9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(AGPAT9 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).)

Western Blot (WB) (AGPAT9 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).)

Western Blot (WB)

(AGPAT9 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).)

Western Blot (WB) (AGPAT9 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).)
Related Product Information for anti-AGPAT9 antibody
Glycerol-3-phosphate (G3P) acyltransferases (GPAT; EC
2.3.1.15), such as GPAM (MIM 602395) and GPAT3, catalyze the
initial step of de novo triacylglycerol (TAG) synthesis by
converting glycerol-3-phosphate (G3P) to lysophosphatidic acid
(LPA) (Cao et al., 2006 [PubMed 17170135]).
Product Categories/Family for anti-AGPAT9 antibody
References
Shan, D., et al. J. Lipid Res. 51(7):1971-1981(2010)
Cao, J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(52):19695-19700(2006)
Tang, W., et al. J. Biochem. Mol. Biol. 39(5):626-635(2006)
Yamada, S., et al. Oncogene 23(35):5901-5911(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48705
NCBI Official Full Name
glycerol-3-phosphate acyltransferase 3
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 9
NCBI Official Symbol
AGPAT9
NCBI Official Synonym Symbols
MAG1; GPAT3; AGPAT8; AGPAT 10; HMFN0839; LPAAT-theta
NCBI Protein Information
glycerol-3-phosphate acyltransferase 3
UniProt Protein Name
Glycerol-3-phosphate acyltransferase 3
UniProt Gene Name
AGPAT9
UniProt Synonym Gene Names
GPAT3; MAG1; GPAT-3; AGPAT 10; 1-AGP acyltransferase 9; 1-AGPAT 9; hGPAT3; LPAAT-theta
UniProt Entry Name
GPAT3_HUMAN

NCBI Description

This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]

Uniprot Description

AGPAT9: Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Membrane protein, multi-pass; EC 2.3.1.51; Transferase; EC 2.3.1.15; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q21.23

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: glycerol-3-phosphate O-acyltransferase activity; 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; regulation of TOR signaling pathway

Research Articles on AGPAT9

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Product Notes

The AGPAT9 agpat9 (Catalog #AAA9201013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 194-222. The AGPAT9 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's AGPAT9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the AGPAT9 agpat9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGPAT9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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