Western Blot (WB)
(The Neurogenin3 (N-term) polyclonal antibody is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.)
Rabbit anti-Human, mouse Neurogenin3 Polyclonal Antibody | anti-NEUROG3 antibody
Neurogenin3 Antibody (N-term)
WB~~1:2000
IHC-P~~1:25
Western Blot (WB)
(The Neurogenin3 (N-term) polyclonal antibody is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.)
Western Blot (WB)
(The Neurogenin3 (N-term) polyclonal antibody is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.)
Western Blot (WB)
(Neurogenin3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Neurogenin3 antibody detected the Neurogenin3 protein (arrow).)
Western Blot (WB)
(Neurogenin3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Neurogenin3 antibody detected the Neurogenin3 protein (arrow).)
Western Blot (WB)
(Western blot analysis of Neurogenin3 (arrow) using rabbit polyclonal Neurogenin3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Neurogenin3 gene.)
Western Blot (WB)
(Western blot analysis of Neurogenin3 (arrow) using rabbit polyclonal Neurogenin3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Neurogenin3 gene.)
Immunohistochemistry (IHC)
(Neurogenin3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human large intestine followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Neurogenin3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Neurogenin3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human large intestine followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Neurogenin3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(All lanes : Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: LNCap whole cell lysate Lane 3: PANC-1 whole cell lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: LNCap whole cell lysate Lane 3: PANC-1 whole cell lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Neurogenin3 Antibody (N-term) at 1:500 dilution + Human placenta tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Neurogenin3 Antibody (N-term) at 1:500 dilution + Human placenta tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(MBS9200827 staining Neurogenin3 in human small intestine tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9200827 staining Neurogenin3 in human small intestine tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9200827 staining Neurogenin3 in human pancreas tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9200827 staining Neurogenin3 in human pancreas tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).[provided by RefSeq, May 2010]
Uniprot Description
neurogenin 3: Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4). DIAR4 is a characterized by severe, life- threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.
Protein type: DNA-binding; Transcription factor; Cell development/differentiation
Chromosomal Location of Human Ortholog: 10q21.3
Cellular Component: cytoplasm; nucleus
Molecular Function: protein dimerization activity; chromatin DNA binding; double-stranded DNA binding; transcription coactivator activity
Biological Process: nervous system development; central nervous system development; transcription, DNA-dependent; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; endocrine pancreas development; peripheral nervous system development; epithelial cell differentiation; spinal cord development; forebrain development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of neuron differentiation; hindbrain development
Disease: Diarrhea 4, Malabsorptive, Congenital
Research Articles on NEUROG3
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Product Notes
The NEUROG3 neurog3 (Catalog #AAA9200827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 40-69. The Neurogenin3 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neurogenin3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Immunofluorescence (IF). IF~~1:10~50 WB~~1:2000 IHC-P~~1:25. Researchers should empirically determine the suitability of the NEUROG3 neurog3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurogenin3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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