Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-SMO-S611/615 antibody at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Jurkat cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Rabbit anti-Human Phospho-SMO-S611/615 Polyclonal Antibody | anti-SMO-S611/615 antibody
Phospho-SMO-S611/615 Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-SMO-S611/615 antibody at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Jurkat cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-SMO-S611/615 antibody at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Jurkat cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
Uniprot Description
SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.
Protein type: Membrane protein, multi-pass; Oncoprotein; GPCR, Fz/Smo family; Receptor, GPCR; Cell development/differentiation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q32.3
Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; plasma membrane; integral to membrane; caveola
Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; patched binding; drug binding
Biological Process: developmental growth; central nervous system neuron differentiation; ventral midline determination; negative regulation of epithelial cell differentiation; skeletal muscle fiber development; cell fate specification; negative regulation of transcription from RNA polymerase II promoter; thalamus development; Wnt receptor signaling pathway through beta-catenin; anterior/posterior pattern formation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; heart looping; vasculogenesis; activation of hh target transcription factor; neural crest cell migration; dorsoventral neural tube patterning; positive regulation of neuroblast proliferation; smoothened signaling pathway; hair follicle morphogenesis; protein stabilization; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; in utero embryonic development; multicellular organism growth; smoothened signaling pathway in ventral spinal cord patterning; forebrain morphogenesis; negative regulation of hair follicle development; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein import into nucleus; cerebellar cortex morphogenesis; myoblast migration; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Research Articles on SMO-S611/615
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Product Notes
The SMO-S611/615 smo (Catalog #AAA9143816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SMO-S611/615 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-SMO-S611/615 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SMO-S611/615 smo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-SMO-S611/615, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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