Rabbit UBQLN3 Polyclonal Antibody | anti-UBQLN3 antibody

UBQLN3 Rabbit pAb

Cat. Num. AAA9142942

• Gene Names: UBQLN3; TUP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity purification
• Synonyms: UBQLN3; Polyclonal Antibody; UBQLN3 Rabbit pAb; TUP-1; anti-UBQLN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence: WTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQL

• Applicable Applications for anti-UBQLN3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 306-620 of human UBQLN3 (NP_059509.1).
• Positive Samples: PC-3, Mouse testis, Rat testis, Rat brain
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UBQLN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of rat testis using UBQLN3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of mouse testis using UBQLN3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-UBQLN3 antibody

Background: This gene encodes a ubiquitin-like protein (ubiquilin) that shares a high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and are thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This gene is specifically expressed in the testis. It has been suggested that this gene may regulate cell-cycle progression during spermatogenesis, however, it has been shown that the ortholgous mouse gene is dispensable for embryonic development and spermatogenesis. [provided by RefSeq, Nov 2016]

NCBI and Uniprot Product Information

• NCBI GI #: 8567418
• NCBI GeneID: 50613
• NCBI Accession #: NP_059509.1
• NCBI GenBank Nucleotide #: NM_017481.2
• UniProt Accession #: Q9H347; Q9NRE0
• Molecular Weight: 70,841 Da
• NCBI Official Full Name: ubiquilin-3
• NCBI Official Synonym Full Names: ubiquilin 3
• NCBI Official Symbol: UBQLN3
• NCBI Official Synonym Symbols: TUP-1
• NCBI Protein Information: ubiquilin-3
• UniProt Protein Name: Ubiquilin-3
• Protein Family: Ubiquilin
• UniProt Gene Name: UBQLN3
• UniProt Entry Name: UBQL3_HUMAN

NCBI Description

Summary: This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This gene is specifically expressed in the testis, and proposed to regulate cell-cycle progression during spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

UBQLN3:

Chromosomal Location of Human Ortholog: 11p15