Rabbit anti-Human, Mouse MCM3AP Polyclonal Antibody | anti-MCM3AP antibody

MCM3AP Rabbit pAb

Cat. Num. AAA9142103

• Gene Names: MCM3AP; GANP; SAC3; MAP80
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: MCM3AP; Polyclonal Antibody; MCM3AP Rabbit pAb; GANP; MAP80; SAC3; anti-MCM3AP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence: SSFPVSSAVLGEPFQASKAGVRQGCEEAVSQVEPLPSLMKGLKRKEDQDRSPRRHGHEPAEDSDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQDVFKSNKEVGRLGNKEAKKETGFVESAESDHMAIPGGNQSVLAPSRIPGVNKEEETESREKKEDSLRGTPARQSNRSESTDSLGGLSPSEVTAIQCKNIPDYLNDRTILENHFGKIAKVQRIFTRRSKKLAVVHFFDHASAALARKKGKSLHKDMAIFWHRKKISPNKKPFSLKEKKPGDGEVSPSTEDAPFQHSPLGKAAGRTGAS

• Applicable Applications for anti-MCM3AP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human MCM3AP (NP_003897.2).
• Cellular Location: Cytoplasm, Nucleus, Nucleus envelope, nuclear pore complex, nucleoplasm
• Positive Samples: Jurkat, HeLa, COS-7, NIH/3T3, Mouse brain
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MCM3AP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)

Related Product Information for anti-MCM3AP antibody

Background: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability.

Product Categories/Family for anti-MCM3AP antibody

Cell Biology & Developmental Biology; Cell Cycle; Cell cycle inhibitors; Research; Cell Biology & Developmental Biology; Cell Cycle; Cell differentiation

NCBI and Uniprot Product Information

• NCBI GI #: 19923191
• NCBI GeneID: 8888
• NCBI Accession #: NP_003897.2
• NCBI GenBank Nucleotide #: NM_003906.3
• UniProt Accession #: O60318; Q2M3C1; Q9UMT4
• Molecular Weight: 218,405 Da
• NCBI Official Full Name: 80 kDa MCM3-associated protein
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 3 associated protein
• NCBI Official Symbol: MCM3AP
• NCBI Official Synonym Symbols: GANP; SAC3; MAP80
• NCBI Protein Information: 80 kDa MCM3-associated protein; MCM3 import protein; germinal center-associated nuclear protein; germinal-centre associated nuclear protein; minichromosome maintenance deficient 3-associated protein; MCM3 minichromosome maintenance deficient 3 associated
• UniProt Protein Name: 80 kDa MCM3-associated protein
• Protein Family: Germinal-center associated nuclear protein
• UniProt Gene Name: MCM3AP
• UniProt Synonym Gene Names: GANP; KIAA0572; MAP80
• UniProt Entry Name: MCM3A_HUMAN

NCBI Description

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. [provided by RefSeq, Jul 2008]

Uniprot Description

GANP: is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located. In contrast, the GANP promoter region contains consensus transcription factor binding sites for E2F, E-box, BSAP, and PU.1. MCM3AP interacts with MCM3, which it can acetylate weakly in vivo. GANP is required for efficient mRNA nuclear export: GANP depletion results in the nuclear accumulation of poly(A)+RNA. It interacts via its N-terminal FG repeat domain with the mRNA export factor NXF1. GANP plays a role in the immune response: antibodies from mice deficient for GANP have reduced affinities against T-cell dependent antigens and a lower frequency of variable region somatic mutation. GANP expression is markedly decreased in malignant glioma (MG) with a poor prognosis, consistently lower than that observed in anaplastic astrocytomas. Significant decreases in GANP expression are associated with hyperploidy and chromosomal instability

Protein type: Acetyltransferase; Karyopherin

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nuclear pore; cytosol; nucleus

Molecular Function: DNA binding; transferase activity, transferring acyl groups

Biological Process: mRNA transport; immune system process; protein import into nucleus; DNA replication