Taq DNA Ligase
Taq DNA Ligase
Applications
Cloning Ligation, Seamless Assembly
Synonyms
Taq DNA Ligase; Taq DNA Ligase ligase
Host
E Coli
Form/Format
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 mug/ml BSA, 50% Glycerol, pH 7.4 at 25 degree C
Concentration
40,000 U/ml (varies by lot)
Applicable Applications for Taq DNA Ligase ligase
Cloning Ligation, Seamless Assembly
Source Note
Purified from an E Coli strain containing the cloned ligase gene from Thermus thermophilus HB8.
Unit Definition
(Cohesive End Unit) One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 ug of BstEII-digested lambda DNA in a total reaction volume of 50 ul in 15 minutes at 45 degree C.
Reaction Buffer or Mix
1X Taq DNA Ligase Reaction Buffer:
20 mM Tris-HCl, 25 mM Potassium Acetate, 10 mM Magnesium Acetate, 1 mM NAD, 10 mM DTT, 0.1% Triton X-100, pH 7.6 at 25 degree C
20 mM Tris-HCl, 25 mM Potassium Acetate, 10 mM Magnesium Acetate, 1 mM NAD, 10 mM DTT, 0.1% Triton X-100, pH 7.6 at 25 degree C
Heat Inactivation
No
Preparation and Storage
Store at -20 degree C.
Related Product Information for Taq DNA Ligase ligase
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 degree C - 75 degree C).
It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.
It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.
Similar Products
Product Notes
The Taq DNA Ligase (Catalog #AAA9141677) is a Ligase produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Taq DNA Ligase can be used in a range of immunoassay formats including, but not limited to, Cloning Ligation, Seamless Assembly. Researchers should empirically determine the suitability of the Taq DNA Ligase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Taq DNA Ligase, Ligase" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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