Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HIC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit HIC2 Polyclonal Antibody | anti-HIC2 antibody
HIC2 Polyclonal Antibody
Gene Names
HIC2; HRG22; ZBTB30; ZNF907
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
HIC2; Polyclonal Antibody; HIC2 Polyclonal Antibody; HRG22; ZBTB30; ZNF907; anti-HIC2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
LTAASYLQLPELAALCRRKLKRAGKPFGSGRAGSTGMGRPPRSQRLSTASVIQARYQGLVDGRKGAHAPQELPQAKGSDDELFLGGSNQDSVQGLGRAVCPAGGEAGLGGCSSSTNGSSGGCEQELGLDLSKKSPPLPPATPGPHLTPDDAAQLSDSQHGSPPAASAPPVANSASYSELGGTPDEPMDLEGAEDNHLSLLEAPGGQPRKSLRHSTRKKEWGKKEPVAGSPFERREAGPKGPCPGEEGEGVGDRVP
Sequence Length
615
Applicable Applications for anti-HIC2 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human HIC2
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Nucleus
Positive Samples
U-87MG, Mouse brain, Rat lung
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HIC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HIC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Product Categories/Family for anti-HIC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 64kDa; 66kDa
Observed: 75kDa
Observed: 75kDa
NCBI Official Full Name
hypermethylated in cancer 2 protein
NCBI Official Synonym Full Names
HIC ZBTB transcriptional repressor 2
NCBI Official Symbol
HIC2
NCBI Official Synonym Symbols
HRG22; ZBTB30; ZNF907
NCBI Protein Information
hypermethylated in cancer 2 protein
UniProt Protein Name
Hypermethylated in cancer 2 protein
Protein Family
UniProt Gene Name
HIC2
UniProt Synonym Gene Names
HRG22; KIAA1020; ZBTB30; Hic-2
Uniprot Description
Transcriptional repressor.
Research Articles on HIC2
Similar Products
Product Notes
The HIC2 hic2 (Catalog #AAA9134318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIC2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HIC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the HIC2 hic2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LTAASYLQLP ELAALCRRKL KRAGKPFGSG RAGSTGMGRP PRSQRLSTAS VIQARYQGLV DGRKGAHAPQ ELPQAKGSDD ELFLGGSNQD SVQGLGRAVC PAGGEAGLGG CSSSTNGSSG GCEQELGLDL SKKSPPLPPA TPGPHLTPDD AAQLSDSQHG SPPAASAPPV ANSASYSELG GTPDEPMDLE GAEDNHLSLL EAPGGQPRKS LRHSTRKKEW GKKEPVAGSP FERREAGPKG PCPGEEGEGV GDRVP. It is sometimes possible for the material contained within the vial of "HIC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
