Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HDGFRP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit HDGFRP3 Polyclonal Antibody | anti-HDGFRP3 antibody
HDGFRP3 Polyclonal Antibody
Gene Names
HDGFRP3; HDGF2; HRP-3; HDGF-2; CGI-142
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
HDGFRP3; Polyclonal Antibody; HDGFRP3 Polyclonal Antibody ; HDGF2; HRP-3; HDGF-2; CGI-142; anti-HDGFRP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
203
Applicable Applications for anti-HDGFRP3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:200 - 1:2000, IHC: 1:20 - 1:200, IF: 1:20 - 1:100, IP: 1:20 - 1:50
Immunogen
Recombinant protein of human HDGFRP3
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HDGFRP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HDGFRP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat spinal cord using HDGFRP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat spinal cord using HDGFRP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human uterine cancer using HDGFRP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human uterine cancer using HDGFRP3 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-HDGFRP3 antibody
HDGFRP3 is a protein-coding gene. GO annotations related to this gene include growth factor activity. An important paralog of this gene is HDGF.
Product Categories/Family for anti-HDGFRP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 23kDa
Molecular Weight: 203
Molecular Weight: 203
NCBI Official Full Name
hepatoma-derived growth factor-related protein 3
NCBI Official Symbol
HDGFRP3
NCBI Official Synonym Symbols
HDGF2; HRP-3; HDGF-2; CGI-142
NCBI Protein Information
hepatoma-derived growth factor-related protein 3
UniProt Protein Name
Hepatoma-derived growth factor-related protein 3
Protein Family
UniProt Gene Name
HDGFRP3
UniProt Synonym Gene Names
HDGF2; HRP-3; HDGF-2
UniProt Entry Name
HDGR3_HUMAN
Uniprot Description
HDGFRP3: Enhances DNA synthesis and may play a role in cell proliferation. Belongs to the HDGF family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 15q25.2
Cellular Component: cytosol; nucleoplasm; nucleus
Molecular Function: growth factor activity; microtubule binding; tubulin binding
Biological Process: cell proliferation; microtubule polymerization; negative regulation of microtubule depolymerization; neurite development
Research Articles on HDGFRP3
Similar Products
Product Notes
The HDGFRP3 hdgfrp3 (Catalog #AAA9129259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDGFRP3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HDGFRP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:200 - 1:2000, IHC: 1:20 - 1:200, IF: 1:20 - 1:100, IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the HDGFRP3 hdgfrp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDGFRP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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