Rabbit ARF4 Polyclonal Antibody | anti-ARF4 antibody

ARF4 Polyclonal Antibody

Cat. Num. AAA9128574

• Gene Names: ARF4; ARF2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: ARF4; Polyclonal Antibody; ARF4 Polyclonal Antibody; anti-ARF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 180

• Applicable Applications for anti-ARF4 antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human ARF4
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ARF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Related Product Information for anti-ARF4 antibody

This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4502205
• NCBI GeneID: 378
• NCBI Accession #: NP_001651.1
• NCBI GenBank Nucleotide #: NM_001660.3
• UniProt Accession #: P18085; P21371; B2R7J7
• Molecular Weight: 21kDa
• NCBI Official Full Name: ADP-ribosylation factor 4
• NCBI Official Synonym Full Names: ADP-ribosylation factor 4
• NCBI Official Symbol: ARF4
• NCBI Official Synonym Symbols: ARF2
• NCBI Protein Information: ADP-ribosylation factor 4
• UniProt Protein Name: ADP-ribosylation factor 4
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF4
• UniProt Synonym Gene Names: ARF2
• UniProt Entry Name: ARF4_HUMAN

NCBI Description

This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF4: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: Golgi apparatus; membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; vesicle-mediated transport; phospholipase D activation; cell migration; protein transport; protein amino acid ADP-ribosylation; small GTPase mediated signal transduction; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; brain development; response to axon injury; negative regulation of apoptosis