Rabbit anti-Human, Mouse GSTM3 Polyclonal Antibody | anti-GSTM3 antibody

GSTM3 Polyclonal Antibody

Cat. Num. AAA9128348

• Gene Names: GSTM3; GST5; GSTB; GTM3; GSTM3-3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: GSTM3; Polyclonal Antibody; GSTM3 Polyclonal Antibody; anti-GSTM3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 225

• Applicable Applications for anti-GSTM3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB 1:500 - 1:2000; IHC 1:50 - 1:200
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human GSTM3
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GSTM3 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Related Product Information for anti-GSTM3 antibody

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 23065552
• NCBI GeneID: 2947
• NCBI Accession #: NP_000840.2
• NCBI GenBank Nucleotide #: NM_000849.4
• UniProt Accession #: P21266; O60550; Q96HA3
• Molecular Weight: 27kDa
• NCBI Official Full Name: glutathione S-transferase Mu 3
• NCBI Official Synonym Full Names: glutathione S-transferase mu 3 (brain)
• NCBI Official Symbol: GSTM3
• NCBI Official Synonym Symbols: GST5; GSTB; GTM3; GSTM3-3
• NCBI Protein Information: glutathione S-transferase Mu 3
• UniProt Protein Name: Glutathione S-transferase Mu 3
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM3
• UniProt Synonym Gene Names: GST5; hGSTM3-3
• UniProt Entry Name: GSTM3_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

GSTM3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers. Belongs to the GST superfamily. Mu family.

Protein type: EC 2.5.1.18; Cell development/differentiation; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; enzyme binding; protein homodimerization activity; glutathione binding; glutathione transferase activity

Biological Process: glutathione metabolic process; establishment of blood-nerve barrier; nitrobenzene metabolic process; response to estrogen stimulus; xenobiotic metabolic process; xenobiotic catabolic process