Western Blot (WB)
(Western blot analysis of extracts of JK cells, using Phospho-eNOS-T495 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit anti-Human eNOS-T495 Polyclonal Antibody | anti-NOS3 antibody
Phospho-eNOS-T495 Polyclonal Antibody
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of JK cells, using Phospho-eNOS-T495 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts of JK cells, using Phospho-eNOS-T495 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts of untreated and EGF-treated NIH/3T3 cells, using Phospho-eNOS-T495 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of untreated and EGF-treated NIH/3T3 cells, using Phospho-eNOS-T495 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)
NCBI and Uniprot Product Information
NCBI Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; cytosol; nucleus
Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; nitric-oxide synthase activity; FMN binding; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding
Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; negative regulation of hydrolase activity; regulation of blood pressure; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; nitric oxide biosynthetic process; regulation of blood vessel size; interaction with host; mitochondrion organization and biogenesis; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; nitric oxide mediated signal transduction; smooth muscle hyperplasia; lung development
Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
Research Articles on NOS3
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Product Notes
The NOS3 nos3 (Catalog #AAA9127891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-eNOS-T495 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eNOS-T495 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNOS-T495, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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