Rat mucin 4, cell surface associated ELISA Kit | MUC4 elisa kit

Rat Mucin-4, MUC4 ELISA Kit

Cat. Num. AAA911860

• Reactivity: Rat
• Synonyms: mucin 4; cell surface associated; Rat Mucin-4; MUC4 ELISA Kit; Rat Mucin-4 (MUC4) ELISA kit; HSA276359; tracheobronchial; MUC4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat MUC4. No significant cross-reactivity or interference between rat MUC4 and analogues was observed.
• Sequence Length: 2344

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 0.78 ng/ml -50 ng/ml.
• Sensitivity: 0.195 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for MUC4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 125952521
• UniProt Accession #: Q63661; Q63655; Q63657; Q63659; Q9JIC4
• Molecular Weight: 248,041 Da
• NCBI Official Full Name: Mucin-4
• UniProt Protein Name: Mucin-4
• Protein Family: Mucin
• UniProt Gene Name: Muc4
• UniProt Synonym Gene Names: Smc; MUC-4; ASGP; pSMC; ASGP-1; ASGP-2
• UniProt Entry Name: MUC4_RAT

Uniprot Description

Function: May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248' 1 PublicationManual assertion based on experiment in:Ref.3