Specificity
This assay has high sensitivity and excellent specificity for detection of human KRT23. No significant cross-reactivity or interference between human KRT23 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.9 ng/ml-60 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LAMA4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT23 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT23 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GenBank Nucleotide #
Molecular Weight
202,524 Da
NCBI Official Full Name
laminin subunit alpha-4 isoform 1
NCBI Official Synonym Full Names
laminin, alpha 4
NCBI Official Symbol
LAMA4
NCBI Official Synonym Symbols
LAMA3; CMD1JJ; LAMA4*-1
NCBI Protein Information
laminin subunit alpha-4; laminin alpha 4 chain
UniProt Protein Name
Laminin subunit alpha-4
UniProt Entry Name
LAMA4_HUMAN
NCBI Description
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]
Uniprot Description
LAMA4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Extracellular matrix
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: extracellular matrix; laminin-1 complex; extracellular region; basement membrane; basal lamina
Molecular Function: protein binding; extracellular matrix structural constituent; receptor binding
Biological Process: regulation of cell adhesion; extracellular matrix organization and biogenesis; regulation of embryonic development; cell adhesion; regulation of cell migration
Disease: Cardiomyopathy, Dilated, 1jj
Research Articles on LAMA4
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Product Notes
The Human LAMA4 lama4 (Catalog #
AAA907209) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA907209 ELISA Kit recognizes Human LAMA4.
It is sometimes possible for the material contained within the vial of
"laminin, alpha 4, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
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aspect of this datasheet at
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