Human neuregulin-2 (NRG2) ELISA Kit | NRG2 elisa kit

Human neuregulin-2, NRG2 ELISA Kit

Cat. Num. AAA902536

• Gene Names: NRG2; DON1; HRG2; NTAK
• Reactivity: Human
• Synonyms: neuregulin-2 (NRG2); Human neuregulin-2; NRG2 ELISA Kit; Human neuregulin-2 (NRG2) ELISA kit; Don-1; HRG2; NTAK; divergent of neuregulin-1; neural- and thymus-derived activator for ErbB kinases; NRG2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NRG2. No significant cross-reactivity or interference between human NRG2 and analogues was observed.
• Sequence Length: 784

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: 7.81 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRG2 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRG2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 296531452
• NCBI GeneID: 9542
• NCBI Accession #: NP_001171864.1
• NCBI GenBank Nucleotide #: NM_001184935.1
• UniProt Accession #: O14511
• Molecular Weight: 91,679 Da
• NCBI Official Full Name: pro-neuregulin-2, membrane-bound isoform isoform 7
• NCBI Official Synonym Full Names: neuregulin 2
• NCBI Official Symbol: NRG2
• NCBI Official Synonym Symbols: DON1; HRG2; NTAK
• NCBI Protein Information: pro-neuregulin-2, membrane-bound isoform; pro-NRG2; divergent of neuregulin-1; neural- and thymus-derived activator for ErbB kinases
• UniProt Protein Name: Pro-neuregulin-2, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: NRG2
• UniProt Synonym Gene Names: NTAK; Pro-NRG2; NRG-2; DON-1; NTAK
• UniProt Entry Name: NRG2_HUMAN

NCBI Description

This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq, May 2010]

Uniprot Description

NRG2: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor. Belongs to the neuregulin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: extracellular space; integral to membrane; extracellular region; plasma membrane

Molecular Function: growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; embryonic development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; organ development; innate immune response; signal transduction