Rabbit ISU1 Polyclonal Antibody | anti-ISU1 antibody

Rabbit anti-Schizosaccharomyces pombe (strain 972/ATCC 24843)(Fission yeast) ISU1 Polyclonal Antibody

• Reactivity: Schizosaccharomyces pombe (strain 972/ATCC 24843)(Fission yeast)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: ISU1; Polyclonal Antibody; Rabbit anti-Schizosaccharomyces pombe (strain 972/ATCC 24843)(Fission yeast) ISU1 Polyclonal Antibody; ISU1 Antibody; Iron sulfur cluster assembly protein 1; mitochondrial; Iron sulfur cluster scaffold protein 1; isu1 SPAC227.13c; anti-ISU1 antibody

• Host: Rabbit
• Reactivity: Schizosaccharomyces pombe (strain 972/ATCC 24843)(Fission yeast)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-ISU1 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) isu1 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 19113879
• NCBI GeneID: 2541513
• NCBI Accession #: NP_592967.1
• NCBI GenBank Nucleotide #: NM_001018367.2
• UniProt Accession #: Q9UTC6
• Molecular Weight: 20,616 Da
• NCBI Official Full Name: mitochondrial iron-sulfur cluster assembly scaffold protein Isu1
• NCBI Official Symbol: isu1
• NCBI Protein Information: mitochondrial iron-sulfur cluster assembly scaffold protein Isu1
• UniProt Protein Name: Iron sulfur cluster assembly protein 1, mitochondrial
• Protein Family: Iron sulfur cluster assembly protein
• UniProt Gene Name: isu1

Uniprot Description

Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein isu1. In a second step, the cluster is released from isu1, transferred to a glutaredoxin, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on isu1 depends on the function of the cysteine desulfurase complex nfs1-isd11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH.