Human mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) ELISA Kit | MASP1 elisa kit

Human mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) , MASP1 ELISA Kit

Cat. Num. AAA900234

• Gene Names: MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
• Reactivity: Human
• Synonyms: mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor); Human mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor); MASP1 ELISA Kit; Human mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) (MASP1) ELISA kit; CRARF; CRARF1; DKFZp686I01199; FLJ26383; MASP; MGC126283; MGC126284; PRSS5; RaRF; Ra-reactive factor serine protease p100; manan-binding lectin serine protease-1; mannan-binding lectin serine protease; MASP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MASP1. No significant cross-reactivity or interference between human MASP1 and analogues was observed.
• Sequence Length: 380

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml -200 ng/ml.
• Sensitivity: The minimum detectable dose of human MASP1 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MASP1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 73623026
• NCBI GeneID: 5648
• NCBI Accession #: NP_001027019.1
• NCBI GenBank Nucleotide #: NM_001031849.2
• UniProt Accession #: P48740; O95570; Q68D21; Q8IUV8; Q96RS4; Q9UF09; A8K542; A8K6M1; B4E2L7
• Molecular Weight: 79,247 Da
• NCBI Official Full Name: mannan-binding lectin serine protease 1 isoform 3
• NCBI Official Synonym Full Names: mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
• NCBI Official Symbol: MASP1
• NCBI Official Synonym Symbols: 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
• NCBI Protein Information: mannan-binding lectin serine protease 1; serine protease 5; complement factor MASP-3; Ra-reactive factor serine protease p100; mannose-binding protein-associated serine protease; mannose-binding lectin-associated serine protease 1; complement-activating component of Ra-reactive factor
• UniProt Protein Name: Mannan-binding lectin serine protease 1
• Protein Family: Mannan-binding lectin serine protease
• UniProt Gene Name: MASP1
• UniProt Synonym Gene Names: CRARF; CRARF1; PRSS5; MASP-1; RaRF
• UniProt Entry Name: MASP1_HUMAN

NCBI Description

This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Uniprot Description

MASP1: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Defects in MASP1 are the cause of 3MC syndrome type 1 (3MC1). 3MC1 is a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly arched eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted

Chromosomal Location of Human Ortholog: 3q27-q28

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; protein binding; protein homodimerization activity; serine-type endopeptidase activity; calcium ion binding; calcium-dependent protein binding

Biological Process: receptor-mediated endocytosis; negative regulation of complement activation; innate immune response; complement activation, lectin pathway; proteolysis; complement activation

Disease: 3mc Syndrome 1