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Testing Data (P-peptide-+)

anti-Human FANCA Antibody | anti-FANCA antibody

FANCA (Phospho-Ser1149) Antibody

Gene Names
FANCA; FA; FA1; FAA; FAH; FA-H; FACA; FANCH
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
FANCA; FANCA (Phospho-Ser1149) Antibody; FA; Fa; FA-H; FA1; FAA; FACA; FAH; FANCH; Fanconi anemia group A protein; Fanconi anemia; complementation group A; anti-FANCA antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
FANCA (Phospho-Ser1149) antibody detects endogenous levels of FANCA only when phosphorylated at serine 1149.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1455
Applicable Applications for anti-FANCA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human FANCA around the phosphorylation site of serine 1149 (D-P-SP-L-M).
All Sites
Human: Ser1149
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using FANCA (Phospho-Ser1149) antibody. Western blot analysis of extracts from HELA cells treated with IGF (100ng/ml, 10mins) and HUVEC cells treated with EGF (200ng/ml, 30mins), using FANCA (Phospho-Ser1149) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using FANCA (Phospho-Ser1149) antibody. Western blot analysis of extracts from HELA cells treated with IGF (100ng/ml, 10mins) and HUVEC cells treated with EGF (200ng/ml, 30mins), using FANCA (Phospho-Ser1149) antibody.)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HeLa cells, using FANCA (Phospho-Ser1149) antibody.)

Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HeLa cells, using FANCA (Phospho-Ser1149) antibody.)
References
Lo Ten Foe J.R., Nat. Genet. 14:320-323(1996). Ianzano L., Genomics 41:309-314(1997). Ricke D.O., Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
159,083 Da
NCBI Official Full Name
Fanconi anemia group A protein isoform a
NCBI Official Synonym Full Names
FA complementation group A
NCBI Official Symbol
FANCA
NCBI Official Synonym Symbols
FA; FA1; FAA; FAH; FA-H; FACA; FANCH
NCBI Protein Information
Fanconi anemia group A protein
UniProt Protein Name
Fanconi anemia group A protein
UniProt Gene Name
FANCA
UniProt Synonym Gene Names
FAA; FACA; FANCH; Protein FACA

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Research Articles on FANCA

Similar Products

Product Notes

The FANCA fanca (Catalog #AAA859704) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The FANCA (Phospho-Ser1149) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FANCA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the FANCA fanca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FANCA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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