anti-Human BCL-2 Antibody | anti-BCL-2 antibody

BCL-2 (Phospho-Ser70) Antibody

Cat. Num. AAA856964

• Gene Names: BCL2; Bcl-2; PPP1R50
• Reactivity: Human
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: BCL-2; BCL-2 (Phospho-Ser70) Antibody; anti-BCL-2 antibody

• Reactivity: Human
• Specificity: BCL-2 (phospho-Ser70) antibody detects endogenous levels of BCL-2 only when phos-phorylated at serine 70.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 239

• Application Notes: IHC: 1:50-1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using BCL-2 (phospho-Ser70) antibody.)

Product Categories/Family for anti-BCL-2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Jin Z, Gao F, et al. J Biol Chem 2004 Sep 17; 279(38): 40209-19. Kumar Biswas S, et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34. Huang ST, et al. (2002) FASEB J; 16(8): 825-32. Yamamoto K, et al. (1999) Mol Cell Biol; 19(12): 8469-78.

NCBI and Uniprot Product Information

• NCBI GI #: 72198189
• NCBI GeneID: 596
• NCBI Accession #: NP_000624.2
• NCBI GenBank Nucleotide #: NM_000633.2
• UniProt Accession #: P10415; P10416; Q13842; Q16197; C9JHD5
• Molecular Weight: 22,337 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2 isoform alpha
• NCBI Official Synonym Full Names: BCL2, apoptosis regulator
• NCBI Official Symbol: BCL2
• NCBI Official Synonym Symbols: Bcl-2; PPP1R50
• NCBI Protein Information: apoptosis regulator Bcl-2
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2

NCBI Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).