Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p56Dok-2 (phospho-Tyr299) antibody.)

anti-Human p56Dok-2 Antibody | anti-p56Dok-2 antibody

p56Dok-2 (Phospho-Tyr299) Antibody

Gene Names
DOK2; p56DOK; p56dok-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
p56Dok-2; p56Dok-2 (Phospho-Tyr299) Antibody; anti-p56Dok-2 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
p56Dok-2 (phospho-Tyr299) antibody detects endogenous levels of p56Dok-2 only when phosphorylated at tyrosine 299.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
412
Applicable Applications for anti-p56Dok-2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V).
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p56Dok-2 (phospho-Tyr299) antibody.)

Immunohistochemistry (IHC) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p56Dok-2 (phospho-Tyr299) antibody.)

Western Blot (WB)

(Starvation - - - + Peptide - + - - Western blot analysis of extracts from K562 cells, using p56Dok-2 (Ab-299) antibody and p56Dok-2 (phospho-Tyr299) antibody.)

Western Blot (WB) (Starvation - - - + Peptide - + - - Western blot analysis of extracts from K562 cells, using p56Dok-2 (Ab-299) antibody and p56Dok-2 (phospho-Tyr299) antibody.)
References
Feng Cong, et, al. (1999) Mol. Cell. Biol; 19: 8314-8325. Serge Lemay, et, al. (2000) Mol. Cell. Biol; 20: 2743-2754. Ute Schaeper, et, al.(2000) J. Cell Biol; 149: 1419. Miyuki Honma, et, al. (2006) Genes Cells; 11: 143-151.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,379 Da
NCBI Official Full Name
docking protein 2 isoform a
NCBI Official Synonym Full Names
docking protein 2
NCBI Official Symbol
DOK2
NCBI Official Synonym Symbols
p56DOK; p56dok-2
NCBI Protein Information
docking protein 2
UniProt Protein Name
Docking protein 2
UniProt Gene Name
DOK2

NCBI Description

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]

Uniprot Description

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation ().

Research Articles on p56Dok-2

Similar Products

Product Notes

The p56Dok-2 dok2 (Catalog #AAA856924) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The p56Dok-2 (Phospho-Tyr299) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p56Dok-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1:1000 IHC: 1:50~1:100. Researchers should empirically determine the suitability of the p56Dok-2 dok2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p56Dok-2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.