anti-Human G3BP-1 Antibody | anti-G3BP-1 antibody

G3BP-1 (Phospho-Ser232) Antibody

Cat. Num. AAA854285

• Gene Names: G3BP1; G3BP; HDH-VIII
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: G3BP-1; G3BP-1 (Phospho-Ser232) Antibody; anti-G3BP-1 antibody

• Reactivity: Human
• Specificity: G3BP-1 (phospho-Ser232) antibody detects endogenous levels of G3BP-1 only when phosphorylated at serine 232.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 466

• Applicable Applications for anti-G3BP-1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (phospho-Ser232) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells using G3BP-1 (Ab-232) antibody (Line 1 and 2) and G3BP-1 (phospho-Ser232) antibody (Line 3 and 4).)

Product Categories/Family for anti-G3BP-1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Tao WA, et al. (2005) Nat Methods. 2(8): 591-598. Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135. Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

NCBI and Uniprot Product Information

• NCBI GI #: 5031703
• NCBI GeneID: 10146
• NCBI Accession #: NP_005745.1
• NCBI GenBank Nucleotide #: NM_005754.2
• UniProt Accession #: Q13283; Q5HYE9
• Molecular Weight: 13,874 Da
• NCBI Official Full Name: ras GTPase-activating protein-binding protein 1
• NCBI Official Synonym Full Names: G3BP stress granule assembly factor 1
• NCBI Official Symbol: G3BP1
• NCBI Official Synonym Symbols: G3BP; HDH-VIII
• NCBI Protein Information: ras GTPase-activating protein-binding protein 1
• UniProt Protein Name: Ras GTPase-activating protein-binding protein 1
• UniProt Gene Name: G3BP1
• UniProt Synonym Gene Names: G3BP; G3BP-1; hDH VIII

NCBI Description

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.