Mouse anti-Human Prostate-Specific Membrane Antigen (PSMA) Monoclonal Antibody | anti-PSMA antibody

Prostate-Specific Membrane Antigen (PSMA) (PT1653) Mouse mAb

Cat. Num. AAA8541250

• Gene Names: FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Prostate-Specific Membrane Antigen (PSMA); Monoclonal Antibody; Prostate-Specific Membrane Antigen (PSMA) (PT1653) Mouse mAb; anti-PSMA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Sequence Length: 750

• Applicable Applications for anti-PSMA antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:100-500; WB: 1:500-2000
• Immunogen: Synthesized peptide derived from human Prostate-Specific Membrane Antigen (PSMA)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 548615
• NCBI GeneID: 2346
• UniProt Accession #: Q04609
• NCBI Official Full Name: Glutamate carboxypeptidase 2
• NCBI Official Synonym Full Names: folate hydrolase 1
• NCBI Official Symbol: FOLH1
• NCBI Official Synonym Symbols: PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• NCBI Protein Information: glutamate carboxypeptidase 2
• UniProt Protein Name: Glutamate carboxypeptidase 2
• UniProt Gene Name: FOLH1
• UniProt Synonym Gene Names: FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA
• UniProt Entry Name: FOLH1_HUMAN

NCBI Description

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

Uniprot Description

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.17.21; Protease

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: membrane; integral to plasma membrane; cytoplasm

Molecular Function: peptidase activity; dipeptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding

Biological Process: folic acid and derivative metabolic process; proteolysis